What Is Bifidobacterium longum 35624 and Why Is It in Align Probiotics?

In recent years, probiotics have surged in popularity as people increasingly seek solutions that naturally help support digestive health. Among the wide range of probiotic strains on the market, Bifidobacterium longum 35624™ stands out for its clinical background, health benefits, and use in #1 doctor and #1 gastroenterologist recommended Align Probiotics. But what exactly is Bifidobacterium longum 35624™ and what makes it a valuable choice in digestive health support?

Bifidobacterium longum 35624™ is a specific strain of probiotic bacteria, isolated from the gastrointestinal tract of a healthy human. This unique and trademarked strain is the single active strain in Align’s flagship 24/7 Digestive Support Probiotic* §.

B. longum 35624 has been uniquely identified and studied, fulfilling international standards for probiotic evaluation, and meeting the International Scientific Association for Probiotics and Prebiotics definition of a probiotic. It’s shown its ability to:

• Survive the acidic environment of the stomach.
• Adhere to intestinal lining.
• Positively interact with the gut microbiota.

The benefits of probiotics are not universal across species or even within the same species Bifidoacteria, Bifidobacterium longum 35624’s studied benefits only apply to it. According to World Gastroenterology Organization, probiotic effects are strain-specific. That means a benefit shown for one strain (like B. longum 35624) cannot be assumed for another strain, even if it's from the same species. The strain, is the unique part of the name in this case it is the numbers at the end, 35624.

Think of probiotic strains like breeds of dogs. Just as all dogs are the same species (Canis familiaris), each breed has unique traits—some excel at guarding, others at herding. Similarly, each probiotic strain has its own set of characteristics and health benefits.

Align’s Bifidobacterium 35624™ strain was developed by gastroenterologist. It supports digestive health, particularly in helping manage occasional:*

• Bloating
• Gas
• Abdominal discomfort

Rather than combining multiple strains, Align focuses on a single, well-studied probiotic strain. This strategic choice is based on the premise that the 35624 is a well-documented strain.

Align Probiotics ensures:

• The product is backed by more than 25 years of research.
• The CFU (colony-forming unit) count matches the amount studied in the clinical trials.
• The strain was selected for its ability to reach and work in the gut.

Our digestive tract contains trillions of microorganisms—collectively known as the gut microbiota — which play a critical role in digestion, immunity, and even mood. However, stress, diet, antibiotics, and illness can disrupt this delicate balance.

Probiotics like B. longum 35624 work by:

• Adding healthy bacteria to the gut
• Promoting digestive balance*
• Helping with occasional digestive disturbances*

These effects may contribute to a healthy gut environment and improved quality of life, especially for individuals experiencing occasional GI upset. After taking Align for 3 weeks 4 out of 5 people reported feeling more confident in their digestive system.^<

Yes. Clinical research has found B. longum 35624 to be well-tolerated with minimal side effects. In controlled trials, adverse reactions were rare and similar to those seen with a placebo. For most healthy adults, the strain poses no risk when taken as directed.

That said, individuals with compromised immune systems those taking certain medications (such as corticosteroids or immune-suppressing drugs), those with serious medical conditions, and pregnant/breastfeeding individuals should consult a healthcare provider before adding any probiotic supplement to their routine.

Align Probiotics is a trusted choice for those seeking a research-based probiotic to help with occasional bloating & gas.* By focusing on one well-researched strain—Bifidobacterium longum 35624™—Align is a reliable brand that delivers digestive support in a convenient, once-daily capsule.

If you're looking for a probiotic that:

• Is backed by over 25 years of research
• Contains a strain with multiple digestive benefits*
• Developed by leading gastroenterologists
• #1 Doctor and Gastroenterologist recommended probiotic brand‡

then Align Probiotics with Bifidobacterium longum 35624™ may be the ideal addition to your wellness routine. If you have questions or underlying health conditions talk to your doctor to see if Align is right for you.

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Altmann, Friedrich et al. “Genome Analysis and Characterisation of the Exopolysaccharide Produced by Bifidobacterium longum subsp. longum 35624™.” PloS one vol. 11,9 e0162983. 22 Sep. 2016, doi:10.1371/journal.pone.0162983 https://pmc.ncbi.nlm.nih.gov/articles/PMC5033381/

Ringel-Kulka, Tamar et al. “Multi-Center, Double-Blind, Randomized, Placebo-Controlled, Parallel-Group Study to Evaluate the Benefit of the Probiotic Bifidobacterium infantis 35624 in Non-Patients With Symptoms of Abdominal Discomfort and Bloating.” The American journal of gastroenterology vol. 112,1 (2017): 145-151. doi:10.1038/ajg.2016.511

https://journals.lww.com/ajg/abstract/2017/01000/multi_center,_double_blind,_randomized,.29.aspx Pubmed National Institue of Health: Search Bifidobacteria 35624 https://pubmed.ncbi.nlm.nih.gov/?term=Bifidobacteria+35624+&sort=pubdate&size=50 (5-29-25) 6/24/25

Hill, C., Guarner, F., Reid, G. et al. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11, 506–514 (2014). https://doi.org/10.1038/nrgastro.2014.66

https://www.nature.com/articles/nrgastro.2014.66

Guarner, Francisco MD*; Sanders, Mary Ellen PhD†; Szajewska, Hania MD, PhD‡; Cohen, Henry MD, MWGO§; Eliakim, Rami MD∥; Herrera-deGuise, Claudia MD¶; Karakan, Tarkan MD#; Merenstein, Dan MD**; Piscoya, Alejandro MD, MSc(Ed), AGAF††; Ramakrishna, Balakrishnan MD‡‡; Salminen, Seppo MSc, MS, PhD§§; Melberg, Jim∥∥. World Gastroenterology Organisation Global Guidelines: Probiotics and Prebiotics. Journal of Clinical Gastroenterology 58(6):p 533-553, July 2024. | DOI: 10.1097/MCG.0000000000002002

https://journals.lww.com/jcge/fulltext/2024/07000/world_gastroenterology_organisation_global.2.aspx

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“Probiotics: Fact Sheet for Consumers” National Institute of Health. November 3, 2023, https://ods.od.nih.gov/factsheets/Probiotics-Consumer/ 6/24/2025